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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOH Recombinant antibody (84943-2-RR)

    WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84943-2-RR (APOH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1, Human APOH Recombinant Matched Antibody Pair - PBS only. 84943-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3084. 84943-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Plasma of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 51.16 µg/mL with a range of 38.03 - 72.18 µg/mL.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    The mean APOH concentration was determined to be 21.74 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Serum of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 69.62 µg/mL with a range of 55.50 - 88.74 µg/mL.

  • Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1, APOH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84943-1-PBS. Detection antibody: 84943-2-PBS. Standard: Eg3084. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84943-2-RR

    Affinity and Kinetic Characterization of 84943-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84943-2-RR against Human APOH were performed. The affinity constant is below 1 pM.

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APOH Recombinant antibody, PBS Only (Detector)

APOH Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84943-2-PBS
Clone No.242454E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84943-2-PBS targets APOH as part of a matched antibody pair:

MP01667-1: 84943-1-PBS capture and 84943-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight38 kDa
Observed molecular weight 43-55 kDa
GenBank accession numberNM_000042.2
Gene Symbol APOH
Gene ID (NCBI) 350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02749
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.