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  • Featured Product
  • KD/KO Validated

APOL2 Polyclonal antibody

APOL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25925-1-AP

Synonyms

Apolipoprotein L-II, ApoL-II, APOL3, APOL II

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using APOL2 Polyclonal antibody (25925-1-AP)

    WB analysis of A549 using 25925-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using APOL2 Polyclonal antibody (25925-1-AP)

    WB analysis of HeLa using 25925-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using APOL2 Polyclonal antibody (25925-1-AP)

    WB analysis of A431 using 25925-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using APOL2 Polyclonal antibody (25925-1-AP)

    IHC staining of human lung cancer using 25925-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using APOL2 Polyclonal antibody (25925-1-AP)

    IHC staining of human lung cancer using 25925-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOL2 Polyclonal antibody (25925-1-AP)

    IF Staining of HeLa using 25925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL2 antibody (25925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25925-1-AP targets APOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOL2 fusion protein Ag7622 相同性解析による交差性が予測される生物種
Full Name apolipoprotein L, 2
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC004395
Gene symbol APOL2
Gene ID (NCBI) 23780
RRIDAB_2880297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APOL2 antibody 25925-1-APDownload protocol
IHC protocol for APOL2 antibody 25925-1-APDownload protocol
IF protocol for APOL2 antibody 25925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Commun (Camb)

Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.

Authors - Haijun Guo
  • KD Validated
View Article
humanWB

Aging (Albany NY)

TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs)

Authors - Jianyun Liu
View Article
human,mouseWB

Nat Chem Biol

A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2

Authors - Lu Gan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Melissa (Verified Customer) (07-29-2022)

This antibody worked well in Huh7 cells. The antibody was used to blot for APOL2 after co-IP for another protein of interest.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Huh7 cells
APOL2 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:25925-1-AP)