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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOL3 Polyclonal antibody (28591-1-AP)

    WB analysis using 28591-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28591-1-AP (APOL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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APOL3 Polyclonal antibody

APOL3 Polyclonal Antibody for WB, ELISA
Cat No. 28591-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ApoL III, APOL3, APOLIII, Apolipoprotein L III, apolipoprotein L, 3, Apolipoprotein L3, CG12 1, CG12_1, TNF inducible protein CG12 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, HCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28591-1-AP targets APOL3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOL3 fusion protein Ag29687 相同性解析による交差性が予測される生物種
Full Name apolipoprotein L, 3
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberNM_145640
Gene Symbol APOL3
Gene ID (NCBI) 80833
RRIDAB_3669663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein L3(APOL3) is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. APOL3 is localized in the cytoplasm and plays a central role in cholesterol transport, affecting the movement of lipids, including cholesterol, and allowing the binding of lipids to organelles.

Protocols

Product Specific Protocols
WB protocol for APOL3 antibody 28591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols