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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOL6 Recombinant antibody

APOL6 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230162E11

Cat no : 82877-2-RR

Synonyms

APOL6, Apolipoprotein L-VI, APOLVI, ApoL-VI, UNQ3095/PRO21341, 230162E11, APOL 6, APOL VI

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOL6 Recombinant antibody (82877-2-RR)

    IF Staining of HeLa using 82877-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL6 antibody (82877-2-RR, Clone: 230162E11 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of U2OS cells using APOL6 Recombinant antibody (82877-2-RR)

    FC experiment of U2OS using 82877-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug APOL6 Recombinant antibody (82877-2-RR, Clone:230162E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82877-2-RR

    Affinity and Kinetic Characterization of 82877-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82877-2-RR against Human APOL6 were performed. The affinity constant is 13.8 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82877-2-RR targets APOL6 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APOL6 fusion protein Ag24430 相同性解析による交差性が予測される生物種
Full Name apolipoprotein L, 6
Calculated molecular weight 38 kDa
GenBank accession numberBC038950
Gene symbol APOL6
Gene ID (NCBI) 80830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for APOL6 antibody 82877-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols