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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOM Polyclonal antibody

APOM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12817-1-AP

Synonyms

NG20, HSPC336, G3A, Apo-M, Apolipoprotein M

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOM Polyclonal antibody (12817-1-AP)

    WB analysis using 12817-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using APOM Polyclonal antibody (12817-1-AP)

    WB analysis of human plasma using 12817-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOM Polyclonal antibody (12817-1-AP)

    IHC staining of human liver using 12817-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOM Polyclonal antibody (12817-1-AP)

    IHC staining of human liver using 12817-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using APOM Polyclonal antibody (12817-1-AP)

    IHC staining of human hepatocirrhosis using 12817-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using APOM Polyclonal antibody (12817-1-AP)

    IHC staining of human hepatocirrhosis using 12817-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOM Polyclonal antibody (12817-1-AP)

    IF Staining of HepG2 using 12817-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, human plasma
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12817-1-AP targets APOM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOM fusion protein Ag3137 相同性解析による交差性が予測される生物種
Full Name apolipoprotein M
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC020683
Gene symbol APOM
Gene ID (NCBI) 55937
RRIDAB_2877884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

Protocols

Product Specific Protocols
WB protocol for APOM antibody 12817-1-APDownload protocol
IHC protocol for APOM antibody 12817-1-APDownload protocol
IF protocol for APOM antibody 12817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Apoptosis

The miR-573/apoM/Bcl2A1-dependent signal transduction pathway is essential for hepatocyte apoptosis and hepatocarcinogenesis.

Authors - Yan-Wei Hu
View Article