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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOM Recombinant antibody (82871-1-RR)

    WB analysis using 82871-1-RR (same clone as 82871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82871-1-RR (APOM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOM Recombinant antibody (82871-1-RR)

    IF Staining of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (82871-1-RR, Clone: 3O19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using APOM Recombinant antibody (82871-1-RR)

    FC experiment of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOM Recombinant antibody (82871-1-RR, Clone:3O19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82871-1-RR

    Affinity and Kinetic Characterization of 82871-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82871-1-RR against Human APOM were performed. The affinity constant is 0.694 nM.

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APOM Recombinant antibody, PBS Only

APOM Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82871-1-PBS
Clone No.3O19

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HSPC336, G3A, Apo-M, Apolipoprotein M, Apo M

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82871-1-PBS targets APOM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APOM fusion protein Ag3048 相同性解析による交差性が予測される生物種
Full Name apolipoprotein M
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC020683
Gene Symbol APOM
Gene ID (NCBI) 55937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95445
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).