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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOOL Polyclonal antibody

APOOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28514-1-AP

Synonyms

apolipoprotein O like, APOOL, CXorf33, FAM121A, MIC27, Protein FAM121A, UNQ8193

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOOL Polyclonal antibody (28514-1-AP)

    WB analysis using 28514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOOL Polyclonal antibody (28514-1-AP)

    IF Staining of HeLa using 28514-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28514-1-AP targets APOOL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOOL fusion protein Ag29392 相同性解析による交差性が予測される生物種
Full Name apolipoprotein O-like
Calculated molecular weight 268 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC107096
Gene symbol APOOL
Gene ID (NCBI) 139322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.

Protocols

Product Specific Protocols
WB protocol for APOOL antibody 28514-1-APDownload protocol
IHC protocol for APOOL antibody 28514-1-APDownload protocol
IF protocol for APOOL antibody 28514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols