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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APPBP2 Polyclonal antibody

APPBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12409-1-AP

Synonyms

APP BP2, APPBP2, HS.84084, KIAA0228, PAT1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using APPBP2 Polyclonal antibody (12409-1-AP)

    WB analysis using 12409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APPBP2 Polyclonal antibody (12409-1-AP)

    WB analysis using 12409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

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Tested Applications

Positive WB detected inA2780 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12409-1-AP targets APPBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPBP2 fusion protein Ag3092 相同性解析による交差性が予測される生物種
Full Name amyloid beta precursor protein (cytoplasmic tail) binding protein 2
Calculated molecular weight 585 aa, 67 kDa
Observed molecular weight67 kDa
GenBank accession numberBC018121
Gene symbol APPBP2
Gene ID (NCBI) 10513
RRIDAB_2918029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).

Protocols

Product Specific Protocols
WB protocol for APPBP2 antibody 12409-1-APDownload protocol
IHC protocol for APPBP2 antibody 12409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
View Article