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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APPL2 Polyclonal antibody

APPL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14294-1-AP

Synonyms

Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    WB analysis of BxPC-3 using 14294-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    WB analysis of BxPC-3 using 14294-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of mouse brain using 14294-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of mouse brain using 14294-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of human malignant melanoma using 14294-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of human pancreas cancer using 14294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    IF Staining of BxPC-3 using 14294-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 14294-1-AP (APPL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inmouse brain tissue, human malignant melanoma tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14294-1-AP targets APPL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPL2 fusion protein Ag5626 相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Calculated molecular weight 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC033731
Gene symbol APPL2
Gene ID (NCBI) 55198
RRIDAB_2878041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APPL2 antibody 14294-1-APDownload protocol
IHC protocol for APPL2 antibody 14294-1-APDownload protocol
IF protocol for APPL2 antibody 14294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cachexia Sarcopenia Muscle

Physical exercise attenuates age-related muscle atrophy and exhibits anti-ageing effects via the adiponectin receptor 1 signalling

Authors - Yuan-Li Chen
View Article
humanWB

J Immunol

Long Noncoding RNA HAFML Promotes Migration and Invasion of Rheumatoid Fibroblast-like Synoviocytes

Authors - Siqi Xu
View Article
mouseIF

J Cell Sci

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region

Authors - Paroma Chatterjee
View Article
mouseIF

bioRxiv

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region

Authors - Paroma Chatterjee
View Article