Validation Data Gallery
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Tested Applications
| Positive WB detected in | BxPC-3 cells |
| Positive IHC detected in | mouse brain tissue, human malignant melanoma tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
14294-1-AP targets APPL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APPL2 fusion protein Ag5626 相同性解析による交差性が予測される生物種 |
| Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 |
| Calculated molecular weight | 74 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC033731 |
| Gene Symbol | APPL2 |
| Gene ID (NCBI) | 55198 |
| RRID | AB_2878041 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEU8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APPL2 antibody 14294-1-AP | Download protocol |
| IHC protocol for APPL2 antibody 14294-1-AP | Download protocol |
| IF protocol for APPL2 antibody 14294-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Physical exercise attenuates age-related muscle atrophy and exhibits anti-ageing effects via the adiponectin receptor 1 signalling | ||
J Immunol Long Noncoding RNA HAFML Promotes Migration and Invasion of Rheumatoid Fibroblast-like Synoviocytes | ||
J Cell Sci GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region | ||
bioRxiv GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region |