Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells, K-562 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
10680-1-AP targets APRIL,TNFSF13 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APRIL,TNFSF13 fusion protein Ag1056 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor (ligand) superfamily, member 13 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC008042 |
| Gene Symbol | TNFSF13 |
| Gene ID (NCBI) | 8741 |
| RRID | AB_2206515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75888 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APRIL,TNFSF13 antibody 10680-1-AP | Download protocol |
| IHC protocol for APRIL,TNFSF13 antibody 10680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol TNFSF13 Is a Novel Onco-Inflammatory Marker and Correlates With Immune Infiltration in Gliomas. | ||
Clin Biochem APRIL knockdown suppresses migration and invasion of human colon carcinoma cells.
| ||
Pathol Oncol Res Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.
|