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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APTX Polyclonal antibody

APTX Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26747-1-AP

Synonyms

Forkhead-associated domain histidine triad-like protein, FHA-HIT, EC:3.6.1.71, AXA1, AXA 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using APTX Polyclonal antibody (26747-1-AP)

    WB analysis of HEK-293T using 26747-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26747-1-AP (APTX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using APTX Polyclonal antibody (26747-1-AP)

    WB analysis of HepG2 using 26747-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26747-1-AP (APTX Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using APTX Polyclonal antibody (26747-1-AP)

    WB analysis of HEK-293T using 26747-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26747-1-AP (APTX Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using APTX Polyclonal antibody (26747-1-AP)

    IHC staining of human ovary cancer using 26747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26747-1-AP (APTX antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using APTX Polyclonal antibody (26747-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26747-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26747-1-AP (APTX antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APTX Polyclonal antibody (26747-1-AP)

    IF Staining of HepG2 using 26747-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APTX antibody (26747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26747-1-AP targets APTX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APTX fusion protein Ag24945 相同性解析による交差性が予測される生物種
Full Name aprataxin
Calculated molecular weight 342 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC104881
Gene symbol APTX
Gene ID (NCBI) 54840
RRIDAB_2880620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APTX, also named as Aprataxin, is a nuclear protein, present in both the nucleoplasm and the nucleolus, which is a member of the histidine triad (HIT) superfamily. APTX is involved in DNA single-strand break repair, mediating protein-protein interactions with molecules responding to DNA damage. APTX contains three conserved domains: an N-terminal forkhead-associated (FHA) domain which mediates protein-protein interactions, a HIT domain that is similar to Hint, and a C-terminal zinc finger domain. Loss of function mutations in APTX, the gene encoding for Aprataxin, destabilize the Aprataxin protein and result in a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal movements of the head and eyes.

Protocols

Product Specific Protocols
WB protocol for APTX antibody 26747-1-APDownload protocol
IHC protocol for APTX antibody 26747-1-APDownload protocol
IF protocol for APTX antibody 26747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell Rep

Base-excision repair pathway regulates transcription-replication conflicts in pancreatic ductal adenocarcinoma

Authors - Fan Meng
View Article