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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AQP2 Polyclonal antibody

AQP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29386-1-AP

Synonyms

ADH water channel, AQP CD, AQP2, AQP-2, Aquaporin 2, aquaporin 2 (collecting duct), Aquaporin CD, WCH CD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using AQP2 Polyclonal antibody (29386-1-AP)

    WB analysis of mouse kidney using 29386-1-AP

    37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29386-1-AP (AQP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using AQP2 Polyclonal antibody (29386-1-AP)

    IHC staining of mouse kidney using 29386-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AQP2 Polyclonal antibody (29386-1-AP)

    IHC staining of human kidney using 29386-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using AQP2 Polyclonal antibody (29386-1-AP)

    IF Staining of mouse kidney using 29386-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), AQP1 antibody (66805-1-Ig, Clone: 2E5D6, green).

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Tested Applications

Positive WB detected in37°C incubated mouse kidney tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29386-1-AP targets AQP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AQP2 fusion protein Ag29931 相同性解析による交差性が予測される生物種
Full Name aquaporin 2 (collecting duct)
Calculated molecular weight 29 kDa
Observed molecular weight28-29, 35-38 kDa
GenBank accession numberNM_000486
Gene symbol AQP2
Gene ID (NCBI) 359
RRIDAB_2918288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.

Protocols

Product Specific Protocols
WB protocol for AQP2 antibody 29386-1-APDownload protocol
IHC protocol for AQP2 antibody 29386-1-APDownload protocol
IF protocol for AQP2 antibody 29386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols