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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aquaporin 4 Monoclonal antibody

Aquaporin 4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G7D3

Cat no : 68448-1-Ig

Synonyms

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AQP4 Monoclonal antibody (68448-1-Ig)

    WB analysis using 68448-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spinal cord tissue using AQP4 Monoclonal antibody (68448-1-Ig)

    WB analysis of rat spinal cord using 68448-1-Ig

    rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aquaporin 4 Monoclonal antibody (68448-1-Ig)

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aquaporin 4 Monoclonal antibody (68448-1-Ig)

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, rat spinal cord tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68448-1-Ig targets Aquaporin 4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9830 相同性解析による交差性が予測される生物種
Full Name Aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight35-37 kDa
GenBank accession numberBC022286
Gene symbol Aquaporin 4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
IHC protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols