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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Aquaporin 4 Recombinant antibody (82296-6-RR)

    WB analysis using 82296-6-RR (same clone as 82296-6-PBS)

    37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Aquaporin 4 Recombinant antibody (82296-6-RR)

    IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Aquaporin 4 Recombinant antibody (82296-6-RR)

    IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Aquaporin 4 Recombinant antibody (82296-6-RR)

    IF Staining of mouse brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (82296-6-RR, Clone: 240856C7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82296-6-RR

    Affinity and Kinetic Characterization of 82296-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

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Aquaporin 4 Recombinant antibody, PBS Only

Aquaporin 4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 82296-6-PBS
Clone No.240856C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82296-6-PBS targets Aquaporin 4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561 相同性解析による交差性が予測される生物種
Full Name Aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 32-34 kDa
GenBank accession numberBC022286
Gene Symbol Aquaporin 4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55087
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.