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  • Featured Product
  • KD/KO Validated

ARC Monoclonal antibody, PBS Only

ARC Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4B5

Cat no : 66550-1-PBS

Synonyms

hArc, Arg3.1, ARC/ARG3.1, Activity-regulated gene 3.1 protein homolog, Activity-regulated cytoskeleton-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARC Monoclonal antibody (66550-1-Ig)

    WB analysis using 66550-1-Ig (same clone as 66550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66550-1-Ig (ARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARC Monoclonal antibody (66550-1-Ig)

    IHC staining of mouse brain using 66550-1-Ig (same clone as 66550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARC Monoclonal antibody (66550-1-Ig)

    IHC staining of mouse brain using 66550-1-Ig (same clone as 66550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ARC Monoclonal antibody (66550-1-Ig)

    IF Staining of SH-SY5Y using 66550-1-Ig (same clone as 66550-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ARC antibody (66550-1-Ig, Clone: 1G4B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66550-1-PBS targets ARC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARC fusion protein Ag9445 相同性解析による交差性が予測される生物種
Full Name activity-regulated cytoskeleton-associated protein
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC012321
Gene symbol ARC
Gene ID (NCBI) 23237
RRIDAB_2881912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.