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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARF3 Polyclonal antibody, PBS Only

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22289-1-PBS

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ARF3 Polyclonal antibody (22289-1-AP)

    WB analysis of mouse brain using 22289-1-AP (same clone as 22289-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using ARF3 Polyclonal antibody (22289-1-AP)

    WB analysis of rat brain using 22289-1-AP (same clone as 22289-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF3 Polyclonal antibody (22289-1-AP)

    IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF3 Polyclonal antibody (22289-1-AP)

    IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF3 Polyclonal antibody (22289-1-AP)

    IF Staining of HeLa using 22289-1-AP (same clone as 22289-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARF3 antibody (22289-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22289-1-PBS targets ARF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 3
Calculated molecular weight 21 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberNM_001659
Gene symbol ARF3
Gene ID (NCBI) 377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562).The antibody is specific to ARF3. If has no cross reaction to other ARF members.