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  • Featured Product
  • KD/KO Validated

ARF4 Polyclonal antibody

ARF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11673-1-AP

Synonyms

ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARF4 Polyclonal antibody (11673-1-AP)

    WB analysis using 11673-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARF4 Polyclonal antibody (11673-1-AP)

    WB analysis of HeLa using 11673-1-AP

    WB result of ARF4 antibody (11673-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ARF4 Polyclonal antibody (11673-1-AP)

    WB analysis using 11673-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARF4 Polyclonal antibody (11673-1-AP)

    WB analysis using 11673-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ARF4 Polyclonal antibody (11673-1-AP)

    IP experiment of mouse brain using 11673-1-AP

    IP result of anti-ARF4 (IP:11673-1-AP, 3ug; Detection:11673-1-AP 1:600) with mouse brain tissue lysate 5200ug.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ARF4 Polyclonal antibody (11673-1-AP)

    IP experiment of HepG2 using 11673-1-AP

    IP result of anti-ARF4 (IP:11673-1-AP, 4ug; Detection:11673-1-AP 1:3000) with HepG2 cells lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF4 Polyclonal antibody (11673-1-AP)

    IHC staining of human breast cancer using 11673-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF4 Polyclonal antibody (11673-1-AP)

    IHC staining of human breast cancer using 11673-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARF4 Polyclonal antibody (11673-1-AP)

    IF Staining of HepG2 using 11673-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11673-1-AP (ARF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using ARF4 Polyclonal antibody (11673-1-AP)

    FC experiment of K-562 using 11673-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (11673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, HepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11673-1-AP targets ARF4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARF4 fusion protein Ag2272 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 4
Calculated molecular weight 180 aa, 21 kDa
Observed molecular weight 16 kDa, 21 kDa
GenBank accession numberBC003364
Gene symbol ARF4
Gene ID (NCBI) 378
RRIDAB_2058463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for ARF4 antibody 11673-1-APDownload protocol
IHC protocol for ARF4 antibody 11673-1-APDownload protocol
IF protocol for ARF4 antibody 11673-1-APDownload protocol
IP protocol for ARF4 antibody 11673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Authors - Reiling Jan H JH
View Article
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
View Article
mouseWB

Nat Commun

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Authors - Hyunho Kim
View Article
humanWB

Proc Natl Acad Sci U S A

Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity.

Authors - Matteo Pasetto
  • KD Validated
View Article
humanWB

Cell Rep

KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum.

Authors - Ming Yuan Li
View Article
humanWB

Oncogene

A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.

Authors - Breege V Howley
View Article