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ARF4 Monoclonal antibody

ARF4 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A4B8

Cat no : 68204-1-Ig

Synonyms

ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4, 2A4B8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis of A549 using 68204-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis of HeLa using 68204-1-Ig

    WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis of HEK-293 using 68204-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARF4 Monoclonal antibody (68204-1-Ig)

    WB analysis of mouse brain using 68204-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARF4 Monoclonal antibody (68204-1-Ig)

    IF Staining of HepG2 using 68204-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using ARF4 Monoclonal antibody (68204-1-Ig)

    FC experiment of K-562 using 68204-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, rat brain tissue, A549 cells, mouse brain tissue, HeLa cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68204-1-Ig targets ARF4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARF4 fusion protein Ag25522 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 4
Calculated molecular weight 180 aa, 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC003364
Gene symbol ARF4
Gene ID (NCBI) 378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for ARF4 antibody 68204-1-IgDownload protocol
IF protocol for ARF4 antibody 68204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols