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  • Featured Product
  • KD/KO Validated

ARF5 Polyclonal antibody

ARF5 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15281-1-AP

Synonyms

ADP-ribosylation factor 5, ADP ribosylation factor 5

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using ARF5 Polyclonal antibody (15281-1-AP)

    WB analysis of PC-12 using 15281-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARF5 Polyclonal antibody (15281-1-AP)

    WB analysis of mouse brain using 15281-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARF5 Polyclonal antibody (15281-1-AP)

    WB analysis of HeLa using 15281-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ARF5 Polyclonal antibody (15281-1-AP)

    WB analysis of human liver using 15281-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ARF5 Polyclonal antibody (15281-1-AP)

    IP experiment of MCF-7 using 15281-1-AP

    IP result of anti-ARF5 (IP:15281-1-AP, 4ug; Detection:15281-1-AP 1:500) with MCF-7 cells lysate 2840 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF5 Polyclonal antibody (15281-1-AP)

    IHC staining of human breast cancer using 15281-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF5 Polyclonal antibody (15281-1-AP)

    IHC staining of human breast cancer using 15281-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF5 Polyclonal antibody (15281-1-AP)

    IF Staining of HeLa using 15281-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15281-1-AP (ARF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15281-1-AP targets ARF5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARF5 fusion protein Ag7400 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 5
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC003043
Gene symbol ARF5
Gene ID (NCBI) 381
RRIDAB_2227435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for ARF5 antibody 15281-1-APDownload protocol
IHC protocol for ARF5 antibody 15281-1-APDownload protocol
IF protocol for ARF5 antibody 15281-1-APDownload protocol
IP protocol for ARF5 antibody 15281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Physiol Biochem

Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma

Authors - Feifeng Song
  • KD Validated
View Article