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  • Featured Product
  • KD/KO Validated

ARF5-Specific Polyclonal antibody

ARF5-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20227-1-AP

Synonyms

ARF5, ADP-ribosylation factor 5, ADP ribosylation factor 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of HepG2 using 20227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of HeLa using 20227-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of mouse kidney using 20227-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of mouse brain using 20227-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of COLO 320 using 20227-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of human testis using 20227-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of mouse colon using 20227-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    WB analysis of mouse liver using 20227-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    IHC staining of human skin cancer using 20227-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using ARF5-Specific Polyclonal antibody (20227-1-AP)

    IHC staining of human stomach using 20227-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF5-Specific Polyclonal antibody (20227-1-AP)

    IF Staining of HeLa using 20227-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, mouse colon tissue, mouse liver tissue, HeLa cells, mouse kidney tissue, mouse brain tissue, COLO 320 cells, human testis tissue
Positive IHC detected inhuman stomach tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20227-1-AP targets ARF5-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 5
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberNM_001662
Gene symbol ARF5
Gene ID (NCBI) 381
RRIDAB_10639517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5.

Protocols

Product Specific Protocols
WB protocol for ARF5-Specific antibody 20227-1-APDownload protocol
IHC protocol for ARF5-Specific antibody 20227-1-APDownload protocol
IF protocol for ARF5-Specific antibody 20227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PeerJ

Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.

Authors - Ruichao Li
View Article
mouseWB

Cell Rep

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis

Authors - Rakhee Bajaj
  • KD Validated
View Article