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KD/KO Validated

ARF6-Specific Polyclonal antibody

Name: ARF6-Specific Polyclonal antibody
Brand: Proteintech
SKU: 20225-1-AP

ARF6-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20225-1-AP

Synonyms

ADP ribosylation factor 6, ARF6, ARF6-Specific

Validation Data Gallery

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AllWBIHC

WB analysis using 20225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20225-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20225-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20225-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 20225-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 20225-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 20225-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 20225-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 20225-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, A549 cells, human liver tissue, MCF-7 cells, HEK-293 cells, rat liver tissue
Positive IHC detected in	human liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 7 publications below

Product Information

20225-1-AP targets ARF6-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor 6
Calculated molecular weight	20 kDa
Observed molecular weight	20 kDa
GenBank accession number	NM_001663
Gene symbol	ARF6
Gene ID (NCBI)	382
RRID	AB_10646481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Protocols

Product Specific Protocols
WB protocol for ARF6-Specific antibody 20225-1-AP	Download protocol
IHC protocol for ARF6-Specific antibody 20225-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles. Authors - James W Clancy View Article
human	WB	Int J Biol Sci Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6 Authors - Xun Lu KD Validated View Article
human	WB	Arterioscler Thromb Vasc Biol BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function. Authors - Lin Sisi S View Article
mouse	WB,IHC	Int J Mol Sci Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells Authors - Kenji Ono View Article
mouse		Sci Rep Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity. Authors - Ermes Candiello View Article
human	IF	PLoS One TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6. Authors - Ouyang Suidong S View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARF6-Specific Polyclonal antibody

ARF6-Specific Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 20225-1-AP

WB analysis of HEK-293 using 20225-1-AP

WB analysis of MCF-7 using 20225-1-AP

WB analysis of HepG2 using 20225-1-AP

WB analysis of mouse liver using 20225-1-AP

WB analysis of A549 using 20225-1-AP

WB analysis of human liver using 20225-1-AP

IHC staining of human liver cancer using 20225-1-AP

IHC staining of human liver cancer using 20225-1-AP

IHC staining of human colon using 20225-1-AP

IHC staining of human colon using 20225-1-AP

IHC staining of human kidney using 20225-1-AP

IHC staining of human kidney using 20225-1-AP

IHC staining of human heart using 20225-1-AP

IHC staining of human heart using 20225-1-AP

IHC staining of human colon using 20225-1-AP

IHC staining of human colon using 20225-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.

Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.