Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
13571-1-AP targets ARFGAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARFGAP1 fusion protein Ag4468 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor GTPase activating protein 1 |
| Calculated molecular weight | 414 aa, 46 kDa |
| Observed molecular weight | 46 kDa, 50 kDa |
| GenBank accession number | BC028233 |
| Gene Symbol | ARFGAP1 |
| Gene ID (NCBI) | 55738 |
| RRID | AB_2058621 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N6T3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARFGAP1 antibody 13571-1-AP | Download protocol |
| IHC protocol for ARFGAP1 antibody 13571-1-AP | Download protocol |
| IF protocol for ARFGAP1 antibody 13571-1-AP | Download protocol |
| IP protocol for ARFGAP1 antibody 13571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.
| ||
FASEB J ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein.
| ||
Vet Microbiol ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. |