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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARFGAP2 Polyclonal antibody

ARFGAP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16519-1-AP

Synonyms

Nbla10535, IRZ, GTPase-activating protein ZNF289, ARFGAP 2, ARF GAP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HepG2 using 16519-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of mouse brain using 16519-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IF Staining of HeLa using 16519-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16519-1-AP targets ARFGAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFGAP2 fusion protein Ag9740 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor GTPase activating protein 2
Calculated molecular weight 521 aa, 57 kDa
Observed molecular weight 57-60 kDa
GenBank accession numberBC030148
Gene symbol ARFGAP2
Gene ID (NCBI) 84364
RRIDAB_2878270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARFGAP2 antibody 16519-1-APDownload protocol
IF protocol for ARFGAP2 antibody 16519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols