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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARFGEF1 Polyclonal antibody (31684-1-AP)

    WB analysis using 31684-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31684-1-AP (ARFGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ARFGEF1 Polyclonal antibody (31684-1-AP)

    IHC staining of human ovary cancer using 31684-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31684-1-AP (ARFGEF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ARFGEF1 Polyclonal antibody

ARFGEF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31684-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293T cells, HeLa cells, NIH/3T3 cells, mouse lung tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31684-1-AP targets ARFGEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFGEF1 fusion protein Ag35629 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Calculated molecular weight208kDa
Observed molecular weight210 kDa
GenBank accession numberNM_001413184
Gene Symbol ARFGEF1
Gene ID (NCBI) 10565
RRIDAB_3670078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Y6D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).

Protocols

Product Specific Protocols
WB protocol for ARFGEF1 antibody 31684-1-APDownload protocol
IHC protocol for ARFGEF1 antibody 31684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols