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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARFGEF2 Recombinant antibody (84296-4-RR)

    WB analysis using 84296-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ARFGEF2 Recombinant antibody (84296-4-RR)

    IHC staining of human stomach cancer using 84296-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ARFGEF2 Recombinant antibody (84296-4-RR)

    IHC staining of human stomach cancer using 84296-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84296-4-RR

    Affinity and Kinetic Characterization of 84296-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

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ARFGEF2 Recombinant antibody

ARFGEF2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84296-4-RR
Clone No.241538H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84296-4-RR targets ARFGEF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARFGEF2 fusion protein Ag35396 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Observed molecular weight202 kDa
GenBank accession numberBC050449
Gene Symbol ARFGEF2
Gene ID (NCBI) 10564
RRIDAB_3671837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6D5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

Protocols

Product Specific Protocols
WB protocol for ARFGEF2 antibody 84296-4-RRDownload protocol
IHC protocol for ARFGEF2 antibody 84296-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols