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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Arfaptin-1 Polyclonal antibody

Arfaptin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 17726-1-AP

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    WB analysis using 17726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17726-1-AP (Arfaptin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    IHC staining of human pancreas using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17726-1-AP (ARFIP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    IHC staining of human pancreas using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17726-1-AP (ARFIP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    IF Staining of HepG2 using 17726-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Arfaptin-1 antibody (17726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Arfaptin-1 Polyclonal antibody (17726-1-AP)

    FC experiment of HepG2 using 17726-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Arfaptin-1 Polyclonal antibody (17726-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inBxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17726-1-AP targets Arfaptin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arfaptin-1 fusion protein Ag12122 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 42 kDa, 38 kDa, 22 kDa
GenBank accession numberBC103759
Gene symbol Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2227457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IHC protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IF protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
View Article