Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 14548-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14548-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14548-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14548-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14548-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14548-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 14548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 14548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 14548-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.
IHC staining of human pancreas using 14548-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.
IF Staining of HeLa using 14548-1-AP
Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 14548-1-AP
Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14548-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected in	human liver tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

14548-1-AP targets ARFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARFIP2 fusion protein Ag6076 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor interacting protein 2
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa, 45 kDa
GenBank accession number	BC000392
Gene Symbol	ARFIP2
Gene ID (NCBI)	23647
RRID	AB_2242930
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53365
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-AP	Download protocol
IHC protocol for ARFIP2 antibody 14548-1-AP	Download protocol
IF protocol for ARFIP2 antibody 14548-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Signal Deciphering the immunological and prognostic features of hepatocellular carcinoma through ADP-ribosylation-related genes analysis and identify potential therapeutic target ARFIP2 Authors - Fenfen Jiang View Article
human,mouse	IHC,WB,IF	J Hepatocell Carcinoma ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway Authors - Kaida Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14548-1-AP

WB analysis of human brain using 14548-1-AP

WB analysis of HeLa using 14548-1-AP

WB analysis of mouse pancreas using 14548-1-AP

WB analysis of human liver using 14548-1-AP

WB analysis of human heart using 14548-1-AP

IHC staining of human liver using 14548-1-AP

IHC staining of human liver using 14548-1-AP

IHC staining of human pancreas using 14548-1-AP