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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14548-1-AP

Synonyms

POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human brain using 14548-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of mouse pancreas using 14548-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human liver using 14548-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human heart using 14548-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    IF Staining of HeLa using 14548-1-AP

    Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    IF Staining of HepG2 using 14548-1-AP

    Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14548-1-AP targets ARFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFIP2 fusion protein Ag6076 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 2
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 45 kDa
GenBank accession numberBC000392
Gene symbol ARFIP2
Gene ID (NCBI) 23647
RRIDAB_2242930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-APDownload protocol
IHC protocol for ARFIP2 antibody 14548-1-APDownload protocol
IF protocol for ARFIP2 antibody 14548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

Deciphering the immunological and prognostic features of hepatocellular carcinoma through ADP-ribosylation-related genes analysis and identify potential therapeutic target ARFIP2

Authors - Fenfen Jiang
View Article
human,mouseIHC,WB,IF

J Hepatocell Carcinoma

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway

Authors - Kaida Huang
View Article