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KD/KO Validated

ARG2 Polyclonal antibody

Name: ARG2 Polyclonal antibody
Brand: Proteintech
SKU: 14825-1-AP

ARG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14825-1-AP

Synonyms

ARG2, Arginase 2, mitochondrial, arginase, type II, Kidney type arginase, Non hepatic arginase, Type II arginase

Validation Data Gallery

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AllWBIHC

WB analysis using 14825-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (ARG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 14825-1-AP
WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARG2 transfected Caco-2 cells.
WB analysis of HEK-293 using 14825-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (ARG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 14825-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14825-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14825-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14825-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 14825-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14825-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Caco-2 cells, HEK-293 cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	human prostate cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

14825-1-AP targets ARG2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARG2 fusion protein Ag6609 相同性解析による交差性が予測される生物種
Full Name	arginase, type II
Calculated molecular weight	39 kDa
Observed molecular weight	39-42 kDa
GenBank accession number	BC001350
Gene symbol	ARG2
Gene ID (NCBI)	384
RRID	AB_2058977
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARG2(Arginase-2, mitochondrial) is also named as kidney-type arginase, non-hepatic arginase and belongs to the arginase family. It may play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis.

Protocols

Product Specific Protocols
WB protocol for ARG2 antibody 14825-1-AP	Download protocol
IHC protocol for ARG2 antibody 14825-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Circulation Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages. Authors - Paul A Mueller View Article
mouse	WB	J Lipid Res Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice. Authors - Hagai Tavori View Article
mouse	WB	Biomed Pharmacother PMID: 30889486 Authors - Lu Gao View Article
mouse	WB	Biochim Biophys Acta Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model. Authors - Terry Jo V Bichell View Article
	IHC	Biol Reprod Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period. Authors - Katherine M Halloran View Article
human	WB	Life Sci Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics. Authors - Rong Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARG2 Polyclonal antibody

ARG2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 14825-1-AP

WB analysis of Caco-2 using 14825-1-AP

WB analysis of HEK-293 using 14825-1-AP

IHC staining of human prostate cancer using 14825-1-AP

IHC staining of human prostate cancer using 14825-1-AP

IHC staining of human prostate cancer using 14825-1-AP

IHC staining of human kidney using 14825-1-AP

IHC staining of human prostate cancer using 14825-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages.

Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice.

PMID: 30889486

Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.

Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.

Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.