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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARH Monoclonal antibody

ARH Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D1B11

Cat no : 66932-2-Ig

Synonyms

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARH Monoclonal antibody (66932-2-Ig)

    WB analysis using 66932-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (ARH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using ARH Monoclonal antibody (66932-2-Ig)

    WB analysis using 66932-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (LDLRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using ARH Monoclonal antibody (66932-2-Ig)

    FC experiment of HeLa using 66932-2-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ARH Monoclonal antibody (66932-2-Ig, Clone:1D1B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66932-2-Ig targets ARH in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARH fusion protein Ag28608 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein receptor adaptor protein 1
Calculated molecular weight 308 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC029770
Gene symbol ARH
Gene ID (NCBI) 26119
RRIDAB_2882258
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARH antibody 66932-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Leukocyte immunoglobulin-like receptor B1 (LILRB1) protects human multiple myeloma cells from ferroptosis by maintaining cholesterol homeostasis

Authors - Miao Xian
  • KD Validated
View Article