Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11940-1-AP targets ARHGAP15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARHGAP15 fusion protein Ag2538 相同性解析による交差性が予測される生物種 |
| Full Name | Rho GTPase activating protein 15 |
| Calculated molecular weight | 475 aa, 55 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC016701 |
| Gene Symbol | ARHGAP15 |
| Gene ID (NCBI) | 55843 |
| RRID | AB_874081 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53QZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARHGAP15 (Rho GTPase Activating Protein 15) is a Rac1-specific GAP. Diseases associated with ARHGAP15 include Diverticulitis and Meier-Gorlin Syndrome 2. Among its related pathways are Signaling by Rho GTPases and RAC2 GTPase cycle. ARHGAP15 deregulation is implicated in many abnormalities (PMID: 29867200). ARHGAP15 decreased in glioma, which promoted the aggressive phenotypes of glioma cells through the activation of Rac19. Intronic mutation of ARHGAP15 is associated with diverticular disease. In addition, ARHGAP15 was a prognosis-related biomarker for pancreatic ductal adenocarcinoma (PMID: 28979141).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| IHC protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| IF protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area. | ||
Cancer Sci Forkhead box P3 regulates ARHGAP15 expression and affects migration of glioma cells through the Rac1 signaling pathway.
| ||
J Neurosci Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. |