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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP18 Polyclonal antibody (28409-1-AP)

    WB analysis using 28409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28409-1-AP (ARHGAP18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP18 Polyclonal antibody (28409-1-AP)

    IF Staining of HeLa using 28409-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28409-1-AP (ARHGAP18 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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ARHGAP18 Polyclonal antibody

ARHGAP18 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Rho-type GTPase-activating protein 18, Rho GTPase-activating protein 18, MacGAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MDA-MB-453s cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28409-1-AP targets ARHGAP18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP18 fusion protein Ag29144 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 18
Calculated molecular weight 663 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC107416
Gene Symbol ARHGAP18
Gene ID (NCBI) 93663
RRIDAB_2881136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP18 antibody 28409-1-APDownload protocol
IF protocol for ARHGAP18 antibody 28409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols