ARHGAP24 Polyclonal antibody

ARHGAP24 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18834-1-AP

Synonyms

ARHGAP24, Filamin A associated RhoGAP, FILGAP, p73, p73RhoGAP, RC GAP72, RCGAP72, Rho type GTPase activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY SAR 88


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Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18834-1-AP targets ARHGAP24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 24
Calculated molecular weight 84 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC098580
Gene symbol ARHGAP24
Gene ID (NCBI) 83478
RRIDAB_10603354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.

Protocols

Product Specific Protocols
IHC protocol for ARHGAP24 antibody 18834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Curr Biol

Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve.

Authors - Russell A Gould
humanWB,IHC

Oncogene

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Authors - Xin Jin
humanIHC

Oncotarget

Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines.

Authors - Darren L Roberts
humanWB

Clin Exp Pharmacol Physiol

ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.

Authors - Li Wang
human,mouseWB,IF

Biochem Biophys Rep

Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways

Authors - Wei Liu