ARHGAP24 Polyclonal antibody
ARHGAP24 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18834-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
18834-1-AP targets ARHGAP24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | Rho GTPase activating protein 24 |
Calculated molecular weight | 84 kDa |
Observed molecular weight | 73 kDa |
GenBank accession number | BC098580 |
Gene symbol | ARHGAP24 |
Gene ID (NCBI) | 83478 |
RRID | AB_10603354 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.
Protocols
Product Specific Protocols | |
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IHC protocol for ARHGAP24 antibody 18834-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Curr Biol Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve. | ||
Oncogene Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma. | ||
Oncotarget Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines. | ||
Clin Exp Pharmacol Physiol ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42. | ||
Biochem Biophys Rep Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways |