• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRAF Polyclonal antibody

GRAF Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17747-1-AP

Synonyms

ARHGAP26, OPHN1L, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of HeLa using 17747-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of mouse thymus using 17747-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of human brain using 17747-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of HepG2 using 17747-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GRAF Polyclonal antibody (17747-1-AP)

    IP experiment of HepG2 using 17747-1-AP

    IP result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of mouse brain using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of mouse brain using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of rat brain using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of rat brain using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human heart using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human heart using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse thymus tissue
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human heart tissue, human liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17747-1-AP targets GRAF in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRAF fusion protein Ag12008 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 26
Calculated molecular weight 759 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC068555
Gene symbol ARHGAP26
Gene ID (NCBI) 23092
RRIDAB_2059284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of GRAF can lead to promyelocytic leukemia, suggesting tumor suppressive activity of GRAF. GRAF was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081). It has been reported that there are three splicing mutants of GRAF, namely GRAF1a (92 kDa),GRAF1b (86 kDa) and GRAF1c (75-82 kDa), and GRAF1b and GRAF1c are the major GRAF1 isoforms in adult brain, whereas GRAF1a is abundant in neonates(PMID: 35624318, PMID: 30626696).

Protocols

Product Specific Protocols
WB protocol for GRAF antibody 17747-1-APDownload protocol
IHC protocol for GRAF antibody 17747-1-APDownload protocol
IP protocol for GRAF antibody 17747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurol

Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis

Authors - Sven Jarius
View Article
humanWB

Brain Res

Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.

Authors - Shufang Zhou
View Article
humanWB,IHC,CoIP

Cell Death Dis

CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway

Authors - Guojie Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Cicek (Verified Customer) (06-11-2024)

I used this antibody on mouse neurons, iPSCs and iNeurons with Western blotting, using beta-actin as positive control. There were specific bands at 90 kDa mark for iPSCs and iNeurons, but not for mouse neurons. It was highly expressed in iNeurons. There were unspecific bands at the 70 kDa mark for all samples.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000