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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRAF Recombinant antibody, PBS Only (Detector)

GRAF Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240086A11

Cat no : 83206-1-PBS

Synonyms

ARHGAP26, 240086A11, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRAF Recombinant antibody (83206-1-RR)

    WB analysis using 83206-1-RR (same clone as 83206-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83206-1-RR (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83206-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00157-1

    Cytometric bead array standard curve of MP00157-1

    Cytometric bead array standard curve of MP00157-1, ARHGAP26 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83206-2-PBS. Detection antibody: 83206-1-PBS. Standard: Ag12008. Range: 0.625-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83206-1-PBS targets GRAF as part of a matched antibody pair:

MP00157-1: 83206-2-PBS capture and 83206-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRAF fusion protein Ag12008 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 26
Calculated molecular weight 759 aa, 86 kDa
Observed molecular weight 92-100 kDa
GenBank accession numberBC068555
Gene symbol ARHGAP26
Gene ID (NCBI) 23092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of ARHGAP26 can lead to promyelocytic leukemia, suggesting tumor suppressive activity of ARHGAP26. ARHGAP26 was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081).