Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16697-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16697-1-AP (ARHGAP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 16697-1-AP
IP result of anti-ARHGAP4 (IP:16697-1-AP, 4ug; Detection:16697-1-AP 1:500) with HL-60 cells lysate 1000ug.
IHC staining of mouse ovary using 16697-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 16697-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 16697-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 16697-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Jurkat using 16697-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Jurkat using 16697-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARHGAP4 Polyclonal antibody

ARHGAP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16697-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RhoGAP4, Rho-GAP hematopoietic protein C1, Rho GTPase-activating protein 4, RGC1, p115

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, Jurkat cells, K-562 cells, human placenta tissue, mouse spleen tissue
Positive IP detected in	HL-60 cells
Positive IHC detected in	human lymphoma tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 3 publications below

Product Information

16697-1-AP targets ARHGAP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARHGAP4 fusion protein Ag10091 相同性解析による交差性が予測される生物種
Full Name	Rho GTPase activating protein 4
Calculated molecular weight	986 aa, 109 kDa
Observed molecular weight	115 kDa
GenBank accession number	BC052303
Gene Symbol	ARHGAP4
Gene ID (NCBI)	393
RRID	AB_1850892
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P98171
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP4 antibody 16697-1-AP	Download protocol
IHC protocol for ARHGAP4 antibody 16697-1-AP	Download protocol
IF protocol for ARHGAP4 antibody 16697-1-AP	Download protocol
IP protocol for ARHGAP4 antibody 16697-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Front Oncol Analysis of ARHGAP4 Expression With Colorectal Cancer Clinical Characteristics and Prognosis. Authors - Ming-Sheng Fu View Article
human	WB,IHC	Life Sci Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology Authors - Xiaoqin Lu View Article
human	IHC	Cancer Biomark Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration Authors - Xuesong Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16697-1-AP

IP experiment of HL-60 using 16697-1-AP

IHC staining of mouse ovary using 16697-1-AP

IHC staining of human lymphoma using 16697-1-AP