Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
55165-1-AP targets ARHGAP5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Rho GTPase activating protein 5 |
| Calculated molecular weight | 172 kDa |
| Observed molecular weight | 170-190 kDa |
| GenBank accession number | NM_001173 |
| Gene Symbol | ARHGAP5 |
| Gene ID (NCBI) | 394 |
| RRID | AB_10858787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13017 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARHGAP5, also named as RHOGAP5 and p190-B, is GTPase-activating protein for Rho family members. It may play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. ARHGAP5 can be directly phosphorylated on a single identified tyrosine residue by the activated INS and IGF-1 receptors. It also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of ARHGAP5 is shown to attenuate signal transducing activity of Rac, Rho and CDC42. The antibody is specific to ARHGAP5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP5 antibody 55165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis. | ||
Nat Metab Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis. |