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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGAP9 Polyclonal antibody, PBS Only

ARHGAP9 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15665-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of Jurkat using 15665-1-AP (same clone as 15665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HL-60 using 15665-1-AP (same clone as 15665-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HeLa using 15665-1-AP (same clone as 15665-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    IF Staining of HeLa using 15665-1-AP (same clone as 15665-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15665-1-AP (ARHGAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15665-1-PBS targets ARHGAP9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP9 fusion protein Ag8104 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 9
Calculated molecular weight 750 aa, 83 kDa
Observed molecular weight 70 kDa, 65 kDa
GenBank accession numberBC006107
Gene symbol ARHGAP9
Gene ID (NCBI) 64333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.