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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGAP9 Polyclonal antibody, PBS Only

ARHGAP9 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15665-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of Jurkat using 15665-1-AP (same clone as 15665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HL-60 using 15665-1-AP (same clone as 15665-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HeLa using 15665-1-AP (same clone as 15665-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    IF Staining of HeLa using 15665-1-AP (same clone as 15665-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15665-1-AP (ARHGAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15665-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15665-1-PBS targets ARHGAP9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP9 fusion protein Ag8104 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 9
Calculated molecular weight 750 aa, 83 kDa
Observed molecular weight 70 kDa, 65 kDa
GenBank accession numberBC006107
Gene symbol ARHGAP9
Gene ID (NCBI) 64333
RRIDAB_2059566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.