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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGDIB Monoclonal antibody (66054-1-Ig)

    WB analysis using 66054-1-Ig (same clone as 66054-1-PBS)

    Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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ARHGDIB Monoclonal antibody, PBS Only

ARHGDIB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66054-1-PBS
Clone No.3E7B7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66054-1-PBS targets ARHGDIB in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARHGDIB fusion protein Ag9091 相同性解析による交差性が予測される生物種
Full Name Rho GDP dissociation inhibitor (GDI) beta
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC009200
Gene Symbol ARHGDIB
Gene ID (NCBI) 397
RRIDAB_11045657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52566
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.