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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGDIG Polyclonal antibody

ARHGDIG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14282-1-AP

Synonyms

ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGDIG Polyclonal antibody (14282-1-AP)

    WB analysis using 14282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARHGDIG Polyclonal antibody (14282-1-AP)

    WB analysis of human brain using 14282-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHGDIG Polyclonal antibody (14282-1-AP)

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHGDIG Polyclonal antibody (14282-1-AP)

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14282-1-AP targets ARHGDIG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGDIG fusion protein Ag5554 相同性解析による交差性が予測される生物種
Full Name Rho GDP dissociation inhibitor (GDI) gamma
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC047699
Gene symbol ARHGDIG
Gene ID (NCBI) 398
RRIDAB_2059733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGDIG antibody 14282-1-APDownload protocol
IHC protocol for ARHGDIG antibody 14282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.

Authors - Hao Bo
View Article