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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis using 67975-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis of HepG2 using 67975-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis of MCF-7 using 67975-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    IF Staining of HeLa using 67975-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67975-1-Ig
Clone No.1D8E3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HepG2 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67975-1-Ig targets ARHGEF16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARHGEF16 fusion protein Ag28675 相同性解析による交差性が予測される生物種
Full Name Rho guanine exchange factor (GEF) 16
Calculated molecular weight 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC002681
Gene Symbol ARHGEF16
Gene ID (NCBI) 27237
RRIDAB_2918724
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VV41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

Protocols

Product Specific Protocols
WB protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
IF protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols