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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGEF18 Polyclonal antibody

ARHGEF18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11243-1-AP

Synonyms

p114-Rho-GEF, p114RhoGEF, P114 RhoGEF, p114 Rho GEF, KIAA0521

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    WB analysis of mouse testis using 11243-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11243-1-AP (ARHGEF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of mouse kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human prostate cancer using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of mouse kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human prostate cancer using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IF Staining of HEK-293 using 11243-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ARHGEF18 antibody (11243-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11243-1-AP targets ARHGEF18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF18 fusion protein Ag1749 相同性解析による交差性が予測される生物種
Full Name rho/rac guanine nucleotide exchange factor (GEF) 18
Calculated molecular weight 131 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC014994
Gene symbol ARHGEF18
Gene ID (NCBI) 23370
RRIDAB_2877753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGEF18 antibody 11243-1-APDownload protocol
IHC protocol for ARHGEF18 antibody 11243-1-APDownload protocol
IF protocol for ARHGEF18 antibody 11243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols