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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ARHGEF19 Recombinant antibody (82966-2-RR)

    IF Staining of U2OS using 82966-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ARHGEF19 antibody (82966-2-RR, Clone: 230290F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of U-2 OS using ARHGEF19 Recombinant antibody (82966-2-RR)

    FC experiment of U-2 OS using 82966-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HeLa cells using ARHGEF19 Recombinant antibody (82966-2-RR)

    FC experiment of HeLa using 82966-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82966-2-RR

    Affinity and Kinetic Characterization of 82966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82966-2-RR against Human ARHGEF19 were performed. The affinity constant is below 1 pM.

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ARHGEF19 Recombinant antibody

ARHGEF19 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82966-2-RR
Clone No.230290F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

WGEF, Ephexin-2, 230290F5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82966-2-RR targets ARHGEF19 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARHGEF19 fusion protein Ag11645 相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 19
Calculated molecular weight 802 aa, 89 kDa
GenBank accession numberBC040640
Gene Symbol ARHGEF19
Gene ID (NCBI) 128272
RRIDAB_3670712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IW93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497).

Protocols

Product Specific Protocols
IF protocol for ARHGEF19 antibody 82966-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols