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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14092-1-AP

Synonyms

KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of PC-12 using 14092-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of Transfected HEK-293 using 14092-1-AP

    WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of Jurkat using 14092-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of HeLa using 14092-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of NIH/3T3 using 14092-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of mouse skeletal muscle using 14092-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    WB analysis of K-562 using 14092-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    IP experiment of HeLa using 14092-1-AP

    IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGEF7 Polyclonal antibody (14092-1-AP)

    IF Staining of HeLa using 14092-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF7 fusion protein Ag5223 相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 7
Calculated molecular weight 803 aa, 90 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC060776
Gene symbol ARHGEF7
Gene ID (NCBI) 8874
RRIDAB_2227567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IF protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IP protocol for ARHGEF7 antibody 14092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.

Authors - Ruth Chia
View Article
humanWB,IHC

Am J Transl Res

Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.

Authors - Tao Ma
View Article