ARHGEF7 Polyclonal antibody
ARHGEF7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14092-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human rectal cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARHGEF7 fusion protein Ag5223 相同性解析による交差性が予測される生物種 |
Full Name | Rho guanine nucleotide exchange factor (GEF) 7 |
Calculated molecular weight | 803 aa, 90 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC060776 |
Gene symbol | ARHGEF7 |
Gene ID (NCBI) | 8874 |
RRID | AB_2227567 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
IHC protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
IF protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
IP protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. | ||
Am J Transl Res Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3. |