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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARID1A Recombinant antibody

ARID1A Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240690F1

Cat no : 83733-3-RR

Synonyms

BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using ARID1A Recombinant antibody (83733-3-RR)

    WB analysis of BxPC-3 using 83733-3-RR

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARID1A Recombinant antibody (83733-3-RR)

    WB analysis using 83733-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARID1A Recombinant antibody (83733-3-RR)

    IF Staining of HeLa using 83733-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARID1A Recombinant antibody (83733-3-RR)

    IF Staining of HeLa using 83733-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83733-3-RR

    Affinity and Kinetic Characterization of 83733-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, BxPC-3 cells, K-562 cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83733-3-RR is ARID1A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARID1A fusion protein Ag30749 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 1A (SWI-like)
Calculated molecular weight 242 kDa
Observed molecular weight250-260 kDa
GenBank accession numberNM_006015
Gene symbol ARID1A
Gene ID (NCBI) 8289
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

Protocols

Product Specific Protocols
WB protocol for ARID1A antibody 83733-3-RRDownload protocol
IF protocol for ARID1A antibody 83733-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols