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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARID2 Polyclonal antibody (23406-1-AP)

    WB analysis using 23406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23406-1-AP (ARID2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARID2 Polyclonal antibody (23406-1-AP)

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARID2 Polyclonal antibody (23406-1-AP)

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ARID2 Polyclonal antibody

ARID2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23406-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23406-1-AP targets ARID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARID2 fusion protein Ag19968 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 2 (ARID, RFX-like)
Calculated molecular weight 1835 aa, 197 kDa
Observed molecular weight200-230 kDa
GenBank accession numberBC090062
Gene Symbol ARID2
Gene ID (NCBI) 196528
RRIDAB_2918080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68CP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARID2 antibody 23406-1-APDownload protocol
IHC protocol for ARID2 antibody 23406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Am Heart Assoc

Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2

Authors - Runtai Chen
View Article