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KD/KO Validated

ARID3A Polyclonal antibody

Name: ARID3A Polyclonal antibody
Brand: Proteintech
SKU: 14068-1-AP

ARID3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14068-1-AP

Synonyms

DRIL1, Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14068-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14068-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 14068-1-AP
IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.
IP experiment of K-562 using 14068-1-AP
IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3680 ug.
IHC staining of human colon cancer using 14068-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14068-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14068-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14068-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14068-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARID3A antibody (14068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IF Staining of HepG2 using 14068-1-AP
Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14068-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, mouse placenta tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

14068-1-AP targets ARID3A in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARID3A fusion protein Ag5124 相同性解析による交差性が予測される生物種
Full Name	AT rich interactive domain 3A (BRIGHT-like)
Calculated molecular weight	63 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC060828
Gene symbol	ARID3A
Gene ID (NCBI)	1820
RRID	AB_2060390
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

Protocols

Product Specific Protocols
WB protocol for ARID3A antibody 14068-1-AP	Download protocol
IHC protocol for ARID3A antibody 14068-1-AP	Download protocol
IF protocol for ARID3A antibody 14068-1-AP	Download protocol
IP protocol for ARID3A antibody 14068-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application Authors - Xiaoxue Ren View Article
mouse	IHC	PLoS Genet Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2. Authors - Blair B Madison View Article
human	WB,IP	Carcinogenesis ARID3A promotes the development of colorectal cancer by upregulating AURKA. Authors - Jing Tang KD Validated View Article
human	WB,IHC	Ann Surg Oncol High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma. Authors - Meiying Song View Article
human,mouse	WB,IF	Cell Biol Int ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells Authors - Xudong Guo KD Validated View Article
human	WB,ChIP	Cell Biol Int ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3. Authors - Yafei Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARID3A Polyclonal antibody

ARID3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14068-1-AP

WB analysis of K-562 using 14068-1-AP

IP experiment of K-562 using 14068-1-AP

IP experiment of K-562 using 14068-1-AP

IHC staining of human colon cancer using 14068-1-AP

IHC staining of human colon cancer using 14068-1-AP

IHC staining of human colon cancer using 14068-1-AP

IHC staining of human colon cancer using 14068-1-AP

IF Staining of HepG2 using 14068-1-AP

IF Staining of HepG2 using 14068-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.

ARID3A promotes the development of colorectal cancer by upregulating AURKA.

High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.

ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells

ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3.