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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ARID3A Monoclonal antibody (68428-1-Ig)

    IF Staining of K-562 using 68428-1-Ig (same clone as 68428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ARID3A antibody (68428-1-Ig, Clone: 2B10C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

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ARID3A Monoclonal antibody, PBS Only

ARID3A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68428-1-PBS
Clone No.2B10C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68428-1-PBS targets ARID3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARID3A fusion protein Ag7655 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 3A (BRIGHT-like)
Calculated molecular weight 63 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC060828
Gene Symbol ARID3A
Gene ID (NCBI) 1820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99856
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).