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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARID4A Recombinant antibody

ARID4A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240436A4

Cat no : 83489-1-RR

Synonyms

RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARID4A Recombinant antibody (83489-1-RR)

    WB analysis using 83489-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83489-1-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ARID4A Recombinant antibody (83489-1-RR)

    IF Staining of MCF-7 using 83489-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ARID4A antibody (83489-1-RR, Clone: 240436A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using ARID4A Recombinant antibody (83489-1-RR)

    FC experiment of U2OS using 83489-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ARID4A Recombinant antibody (83489-1-RR, Clone:240436A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83489-1-RR

    Affinity and Kinetic Characterization of 83489-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83489-1-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, DU 145 cells, LNCaP cells, SW 480 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83489-1-RR is ARID4A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARID4A fusion protein Ag33114 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 4A (RBP1-like)
Calculated molecular weight143 kDa
Observed molecular weight205 kDa
GenBank accession numberNM_002892.3
Gene symbol ARID4A
Gene ID (NCBI) 5926
RRIDAB_3671115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.

Protocols

Product Specific Protocols
WB protocol for ARID4A antibody 83489-1-RRDownload protocol
IF protocol for ARID4A antibody 83489-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols