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Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using ARID4B Monoclonal antibody (67384-1-Ig)

    WB analysis of T-47D using 67384-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-231 cells using ARID4B Monoclonal antibody (67384-1-Ig)

    WB analysis of MDA-MB-231 using 67384-1-Ig

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARID4B Monoclonal antibody (67384-1-Ig)

    IHC staining of human breast cancer using 67384-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARID4B Monoclonal antibody (67384-1-Ig)

    IHC staining of human breast cancer using 67384-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ARID4B Monoclonal antibody

ARID4B Monoclonal Antibody for WB, ELISA
Cat No. 67384-1-Ig
Clone No.1H3E2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inT-47D cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67384-1-Ig targets ARID4B in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARID4B fusion protein Ag21485 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 4B (RBP1-like)
Calculated molecular weight 1312 aa, 148 kDa
GenBank accession numberBC130418
Gene Symbol ARID4B
Gene ID (NCBI) 51742
RRIDAB_2918489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ4LE39
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Protocols

Product Specific Protocols
WB protocol for ARID4B antibody 67384-1-IgDownload protocol
IHC protocol for ARID4B antibody 67384-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols