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  • KD/KO Validated

ARIH1 Polyclonal antibody

ARIH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14949-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

H7 AP2, EC:2.3.2.31, E3 ubiquitin-protein ligase ARIH1, ARI-1, ARI 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14949-1-AP targets ARIH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARIH1 fusion protein Ag6760 相同性解析による交差性が予測される生物種
Full Name ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila)
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC051877
Gene Symbol ARIH1
Gene ID (NCBI) 25820
RRIDAB_3085447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4X5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767).

Protocols

Product Specific Protocols
WB protocol for ARIH1 antibody 14949-1-APDownload protocol
IHC protocol for ARIH1 antibody 14949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity

Authors - Lei Chu
  • KD Validated
humanWB

bioRxiv

MDA5 ISGylation is crucial for immune signaling to control viral replication and pathogenesis

Authors - Lucky Sarkar
  • KD Validated
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